PCR Tests at PDS

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Test Name	Method	Sample Comment	Species
Anthrax PCR	PCR	Fresh blood or swabs
Atypical Porcine Pestivirus PCR (APPV)	PCR	Serum, brain, fecal swab, nasal swab, heart	Porcine
Avian Adenovirus PCR	PCR	Live birds: swabs (cloacal, tracheal). Dead birds: liver, spleen.	Avian (Domestic)
Avian Influenza (AIV) PCR	PCR	Swabs in transport medium	Avian (Domestic), Birds
Avian Influenza (AIV) subtyping H5/H7 (PCR)	PCR	Swabs in transport medium	Avian (Domestic), Birds
Avian metapneumovirus type A, B, C – PCR	PCR	Live: swabs (oropharyngeal, cloacal). Dead: trachea, lung, cecal tonsil.	Avian (Domestic)
Avian Paramyxovirus (NDV) subtyping PCR	PCR	Done on Avian Paramyxovirus Matrix PCR positive samples	Avian (Domestic), Birds
Avian Paramyxovirus 1 (NDV) PCR	PCR	Tissues, swabs	Avian (Domestic), Birds
Avian Reovirus PCR	PCR	Live birds: cloacal swab. Dead birds: closed hocks, sterile joint swab (viral arthritis), liver, spleen, trachea, lung.	Avian (Domestic), Birds
Avibacterium paragallinarum PCR	PCR	Oropharyngeal swab, tracheal/lung/air sac swab, Infraorbiatal sinus exudate, Lung
Bovine Coronavirus and Rotavirus PCR	PCR	Jejunum, ileum, or feces	Bovine
Bovine Coronavirus PCR	PCR	Spiral colon, ileum or feces	Bovine
Bovine Diarrhea Panel	Bacteriology, Parasitology, PCR Bacteriology, Parasitology, PCR Bacteriology, Parasitology, PCR	Feces or Intestine	Bovine
Bovine Papilloma Virus PCR	PCR	Tissues - fresh or formalin fixed, paraffin embeded	Bovine
Bovine Parainfluenza 3 (PI3) PCR	PCR	Lung, nasal swabs or tracheal washings	Bovine
Bovine Respiratory Panel – 6 PCR Targets	PCR	Lung, nasal swabs or tracheal washings	Bovine
Bovine Respiratory Panel – 7 PCR Targets	PCR	Lung, nasal swabs or tracheal washings	Bovine
Bovine Respiratory Panel – 7 PCR Targets + C&S	Bacteriology, PCR Bacteriology, PCR	Lung, nasal swabs or tracheal washings	Bovine
Bovine Respiratory Syncytial Virus (BRSV) PCR	PCR	Lung, nasal swabs or tracheal washings	Bovine
Bovine Rotavirus PCR	PCR	Jejunum, ileum, or feces	Bovine
Bovine Viral Diarrhea Virus (BVD) Individual PCR	PCR	EDTA whole blood - 1 mL (best sample for antemortem transient infection); serum - 1 mL; fresh, unfixed tissue (ileum, lymphoid tissues, kidney, skin). Fixed tissues are not acceptable.	Bovine
Bovine Viral Diarrhea Virus (BVD) Pooled PCR	PCR	Skin biopsy - fresh tissue (fixed tissue is not acceptable); serum - 1 mL from animals > 2 months old. Send individual samples - pooling is done in the lab.	Bovine
Brachyspira hyodysenteriae and pilosicoli PCR	PCR	Spiral colon, intestinal contents, feces or rectal swabs	Porcine
Brachyspira hyodysenteriae PCR	PCR	Spiral colon, intestinal contents, feces or rectal swabs	Porcine
Cache Valley Virus PCR	PCR	Brain, Placenta and swabs in viral transport media	Ovine
Campylobacter fetus ssp fetus PCR	PCR	Tissues	Bovine
Campylobacter fetus ssp venerealis PCR	PCR	Preputial wash/scraping or cervical muscus	Bovine
Campylobacter fetus ssp. venerealis/Tritrichomonas foetus PCR	PCR	Preputial wash/scrapings (In Pouch™ medium)	Bovine
Campylobacter sp. PCR	PCR	Tissues	Bovine
Chlamydophila abortus PCR	PCR	Tissue, abomasal fluid	Bovine, Caprine, Ovine
Chlamydophila psittaci PCR	PCR	Tissue, cloacal or eye swabs	Avian (Domestic)
Classical Swine Fever Virus (CSF) CFIA accredited Test	PCR	Contact PDS Diagnostic Pathologist or PDS lab for details.	Porcine
Clostridium difficile genotyping PCR	PCR	Bacterial Isolates only Additional charges will apply if bacterial culture required.	Avian (Domestic), Bovine, Canine, Caprine, Equine, Feline, Ovine, Porcine
Clostridium perfringens toxin typing PCR	PCR	Bacterial Culture	Avian (Domestic), Bovine, Canine, Caprine, Equine, Feline, Ovine, Porcine
Coxiella burnetti PCR	PCR	Placenta, fetal tissue	Bovine
CWD Genotyping Elk (PCR)	PCR	Blood, tissue, skin	Elk
CWD Genotyping White-tail Deer (PCR)	PCR	Blood, tissue, skin	Deer
E. coli Bovine Enteric Virotyping by Real Time PCR	PCR	Bacterial Isolates only Additional charges will apply if bacterial culture required.	Bovine
E. coli Canine Enteric Virotyping by Real Time PCR	PCR	Bacterial Isolates only Additional charges will apply if bacterial culture required.	Canine
E. coli Enteric Virotyping by Real Time PCR	PCR	Bacterial Isolates only Additional charges will apply if bacterial culture required.	Bovine, Canine, Feline, Porcine
E. coli Porcine Enteric Virotyping by Real Time PCR	PCR	Bacterial Isolates only Additional charges will apply if bacterial culture required.	Porcine
Equine Herpesvirus 1 (EHV1) Genotyping	PCR	Done on EHV 1 PCR positive samples	Equine
Equine Herpesvirus 1 and 4 (EHV1/EHV4) PCR	PCR	Nasal swab, tracheal wash or EDTA whole blood	Equine
Equine Herpesvirus 1 and 4 (EHV1/EHV4) PCR (STAT)	PCR	Nasal swab, tracheal wash or EDTA whole blood	Equine
Equine Respiratory Panel PCR	PCR	Nasal swab or tracheal wash	Equine
Equine Respiratory Panel PCR (STAT)	PCR	Nasal swab or tracheal wash	Equine
Erysipelothrix rhusiopathiae PCR	PCR	Tonsils, heart, joint swab, feces, spleen, lymph nodes	Porcine
Feline Calicivirus/Herpesvirus PCR	PCR	Nasal or oral swabs, lung, trachea or tonsil	Feline
Flax CDC Triffid FP967 (3 day TAT)	PCR	Flax seeds	Feed
Flax CDC Triffid FP967 (Same Day)	PCR	Flax seeds	Feed

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PCR Tests at PDS